AccuPower® Rocketplex RT-PCR PreMix is a new product that is in a class by itself.
It provides you with the best, most thermostable Reverse transcription available, while giving you multiplex capabilities in the subsequent PCR.
By combining Multiplex RT-PCR with RocketScript™ Reverse Transcriptase you can amplify from 1 to more than 10 target genes.
AccuPower® Rocketplex RT-PCR PreMix contains all the components necessary for sequential cDNA synthesis and amplification of multiple targets in one tube (one-step RT-PCR).
The RT-PCR PreMix consists of both RocketScript™ Reverse Transcriptase, an RNA dependent DNA polymerase, and a thermostable DNA polymerase in a lyophilized mix of dNTPs, reaction buffer,
RNase inhibitor, Loading dye and stabilizer.
Note: Schematic representation of the 5' UTR of a gene, with complex secondary structure, at three different temperatures.
Note that RocketScript™ RTase shows full activity at 70°C allowing it to synthesize the complete gene sequence where M-MLV and other Reverse Transcriptase's fail.
Features and Benefits
||Detect 1-10 target genes in a single tube directly from total RNA
| Thermostable Activity:
||Thermostable reverse transcription activity (up to 70°C) lets you blast through secondary Structure
||by using enzyme-mediated HotStart, PCR is highly specific.
| High efficiency and sensitivity:
||Detect every gene, every time!
||Simply add RNA, Primer and distilled water to begin. All other reaction components are included.
||Bioneer's strict quality controlled production system ensures that your results will be reproducible experiment after experiment.