AccuPower® Dual-HotStart™ RT-PCR PreMix (PCT/KR2013001935, US14383793, EP13757504, etc.)
is a breakthrough product to overcome frequent problems from RT reaction.
By using this proprietary product, cDNA synthesis of your selective RNA will be performed with high sensitivity from a small amount of template RNA.
Successive PCR amplification of synthesized cDNA also occurs via a one-step reaction with cDNA synthesis.
This one-step RT-PCR product is easily generated with excellent sensitivity for a wide range of applications,
including several types of virus tests and gene expression analysis experiments. This product is ready-to-use,
simply add template RNA, primers and DEPC-DW, and obtain your results with great reproducibility.
Features and Benefits
Low detection limit with the smallest amount of your target template RNA, even in highly concentrated sample.
Optimized to get accurate target gene amplification by using our premier Dual-HotStart™ RT-PCR reaction which utilizes
Pyro-HotStart RT reaction and HotStart PCR.
Use of various Template RNAs:
This product includes Dual-HotStart™ RTase which can achieve RT reaction in unusually high temperature up to 70°C.
So it is possible to adopt various template RNAs even if they have a complex secondary structure.
This kit contains all the reagents for RT-PCR, including thermostable DNA polymerase, RocketScript™ RTase,
dNTPs and more, in one tube as freeze-and-dried format. User only needs to add template RNA,
primers and DEPC-water into the tube.
Containing all the components in each tube or well for reverse transcription and PCR, it maximizes reproducibility
eliminating any carry-over contamination or technician error.
The proprietary lyophilization format allows 30 days-stable shelf life at ambient temperature and 2 years at -20°C.