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AccuRapid™ Cloning Kit from Bioneer


Overview
Technical/Specs
Literature/Support
Ordering Information
Primer design

Primer design

  To use the AccuRapid™ Cloning Kit, the insert should be amplified and the extension primer used should be designed to contain a 18-21 bp complementary sequence of both ends of the linearized vector at the 5' end of the primer.

ex) When cloning into NdeI (CATATG), XhoI (CTCGAG) enzyme site.

3’ end vector sequence (Forward primer)



5’ end vector sequence (Reverse primer)



Insert sequence1


Insert sequence2


Insert sequence3



Name Sequence bp
Insert-1_FP
Insert-1_RP
Insert-2_FP
Insert-2_RP
Insert-3_FP
Insert-3_RP