ExicyclerTM Real Time PCR (qPCR)
 
Description
 ExicyclerTM96 Quantitative Thermal Block (hereinafter referred as ¡°Exicycler¢â 96¡±) is the combination product of thermal cycling block and Bioneer¡¯s innovative Module of detecting versatile fluorescences.

 It is optimized for measuring gene expression levels, detecting and quantitating pathogens, performing allelic discrimination (SNP genotyping) assays as well as scoring the presence of gene sequences
 
Features & Benefits*

 Exicycler¢â 96 is a leading edge system with features to support labs requiring more capabilities from their real time PCR platform.

Variable excitation and five (5)-color detection enable you to use a broad range of fluorophores
including FAM¢â/SYBR¢ç Green I, VIC¢ç/JOE, NED¢â/ TAMRA¢â/ Cy3¢â, ROX¢â/Texas Red¢ç,
and Cy5¢â dyes for greater assay versatility.
Measure gene expression levels, detect and quantitate pathogens, perform allelic discrimination
(SNP genotyping) assays as well as score the presence of gene sequences.
Supports 96-well format plates and 0.2 ml tubes.
Powerful, versatile software including plate set-up wizards and automated analysis tools make
data processing simple and straightforward.
Data viewing capabilities and automated analysis tools
 
EXPANSION OF EXICITATION/DETECTION CAPABILITIES
 Exicycler¢â 96 is a sophisticated platform for users who require extended capabilities and maximum versatility. This features an innovative optical system that enhances sensitivity and lets you access a broader range of fluorophores including FAM¢â/SYBR¢ç Green I, VIC¢ç/JOE, NED¢â/ TAMRA¢â/ Cy3¢â, ROX¢â/Texas Red¢ç, and Cy5¢â dyes. The variable excitation capability allows greater sensitivity for longer wavelength (red) dyes. This enables to excite and/or emit up to five pairs of fluorophores for supporting a wide variety of applications.
Filter Exi Emi Dye
1 490 520 FAM, SYBR Green
2 520 550 JOE, VIC, HEX, TET
3 550 580 TAMRA, CY3
4 580 610 Taxas Red, ROX, RED610
5 640 670 CY5, RED670
 
FIVE MULTIPLEXING
 Measure changes in gene expression, detect and quantitate pathogens, and perform allelic discrimination (SNP genotyping) assays. You can also confirm the presence or absence of a transgene, or other specific target sequence. To facilitate some of these applications, Bioneer provides preformulated, ready-to-use and quality-tested TaqMan¢ç assay for use with the Exicycler¢â 96. Now you can reduce your assay optimization efforts.
 
HIGH THROUGHPUT

Exicycler¢â 96 conveys the latest generation Peltier-base thermal cycling system, which makes you use both standard 96-well plates and 0.2ml tubes. So, with Exicycler¢â 96, you can process high throughput of samples in 96-well plates and can detect fluorescence of 96 samples simultaneously.

Also, sophisticated and versatile platforms for users requiring extended capabilities feature a unique optical system that give homogeneity by effectively minimizing fluorescence differences illuminated on each well. Therefore, the thermal gradient function makes uniform and exquisite.

Five (5) pairs of optical configuration support a broader range of fluorophores. Precision optics and charge-coupled device (CCD) camera, plus a sophisticated multi-componenting algorithm is capable superior accuracy, reproducibility and reliability with no need for hard ware changes to access new dyes.

This feature enables to be compatible with a robotic instrument and automatic preparation equipment with Exicycler¢â 96¡¯s automated door opening capability.

 
USER FRIENDLY GUI SOFTWARE
 Exicycler¢â 96 features the intuitive graphic use interface (GUI)-based software offers ease of use and functionality, including plate set up wizards guide you through all aspects of experimental set-up for even complicated multiplex assays, including the use of multiple standard curves on a single plate. The real time monitoring feature allows you to watch PCR amplification as it happens. Data analysis is simplified with built-in tools such as auto-baseline, auto-threshold, and SNP auto-calling. Besides, advance data viewing features allow you to visualize, report, and understand your datasets.
(i) The Real time monitoring, Auto-baseline and auto-threshold for simplified
  data analysis as PCR amplification happens.
(ii) Intuitive and flexible Graphic Use Interface (GUI)-based software
A linear standard curve from standard samples ranging 10 to 10 ^8 (or more) in initial concentration
gene expression levels measure, pathogens detection & quantitation, the presence of gene sequences score, and allelic discrimination (SNP genotyping) assays performance etc.
Minimization of CT value variation within CT 0.5 cycle, even any amplifications are performed at or around each well.
 
AFFORDABLE PRICE
 Exicycler¢â 96 is a full-featured instrument that combines high-end results with the most reasonable price. With Exicycler¢â 96, we guarantee the superior data quality and proven reliability.
 
Applications
SNP & Melting Curve Analysis
Automated SNP genotype calling capability with intuitive graphical output and quality-value assignment
Simple dissociation curve data collection and viewing
 
Technical Specifications
Instrument
Dimensions (mm) 470(H) x 355(W) x 540(D)
Weight 30 kg
Thermal Cycling System Peltier-based system
Block Format 96-well block
Compatible Consumables 96-well plates and 0.2 mL tubes
Supported Volumes 20~ 50 ¥ìL
Operating Temperature 15-30 ¡É
Operating Humidity 20-80%, no condensation
Operating System Windows XP
Electrical (Voltage / Frequency) 110V or 220V / 50Hz or 60Hz
External I/O Ports USB 2.0
Thermal Block Specifications
Temperature Range

4¡É - 99 ¡É
Temperature Accuracy +/-0.3
Peak Block Ramp Rate Max 2.5 ¡É/sec
Gradient Range 1~ 20 ¡É
Gradient Temperature Range 40~75 ¡É
Optical Specifications
Optical System UHP short arc lamp (120W)
Five-excitation filters, five-emission filters and 2D CCD camera
Calibrated Dyes at Installation SYBR¢ç Green I, FAM¢â, JOE¢â, TAMRA¢â, ROX¢â, Texas Red¢ç, Cy3¢â, Cy5¢â (490~670mm)
 
* Bioneer is a registered trademark or trademark of Bioneer Corporation. Exicycler is a registered
  trademark in Korea and is trademark in the other countries.
*¡®SYBR¡¯ is a registered trademark of Molecular Probes, Inc.
* ¡®TaqMan¡¯ is a registered trademark of Roche Molecular System, Inc.
* ¡®VIC¡¯ is a registered trademark of Celera Discovery System. FAM, JOE, NED, ROX, and TAMRA are
  trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries.
* All other trademarks are the property of their respective owners.

 

 

 

 Ordering Information


  Product Size Contents Cat No.
  ExicyclerTM Quantitative Thermal Block 1 ea Each A-2060