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MDx Platform Technologies from Bioneer

Molecular diagnostics is an integrated fusion technology of reagents and instruments.

AccuPower®
Enzyme-mediated HotStart
Dual-HotStartTM
RocketScriptTM

Dual-HotStart™ Technology (KR 10-292883)

Figure 1

Figure 1. Principle of Dual-HotStart™ technology

Dual-HotStart™ technology is a method to detect RNA target from clinical samples with high sensitivity and specificity. This technology utilizes Bioneer’s unique enzyme-mediated HotStart method for both reverse transcription and PCR amplification. Dual-HotStart eliminates non-specific cDNA synthesis and non-specific DNA amplification and allows for the most sensitive one-step RT-qPCR assays available.

Performance

Dual-HotStart™ RT-PCR technology enhances specificity and sensitivity by removing non-specific RNA amplification.

Figure 2

Figure 2. High-sensitivity detection of RNA target

Serial dilutions of HCV RNA were amplified by conventional RT-PCR (left panel) or Dual-HotStart™ RT-PCR (right panel). The results suggest that Dual-HotStart™ RT-PCR enables highly sensitive detection of low copies of RNA target by eliminating non-specific cDNA synthesis and non-specific PCR amplification.

Intellectual Property

Title Patent No. Application No. Countries
 Compositions for hot start reverse transcription reaction or hot
 start reverse transcription polymerase chain reaction
  KR 10-2013-0025119 KR
 Compositions for hot start reverse transcription reaction or hot
 start reverse transcription polymerase chain reaction
  PCT/KR2013/001935 PCT