AccuPower® ETEC-Pili 5-Plex PCR Kit provides one-step detection of 5 types of pilis produced by ETEC E. coli in
animals: F4 (K88), F5 (K99), F6 (987P), F18 and F41. This featured kit is comprised of all the essential requirements for
PCR reactions including primer, salts, dNTPs, and stabilizers in one tube to the provivded ready-to-use Premix format
product. Users only need to add template DNA and ddH20 to perform the test.
AccuPower® ETEC-Pili 5-Pplex PCR Kit is optimized to the application in processing multiple diagnostic samples by the
Labchip GX I (Caliper Life Sciences) automated system. Detection limit of this kit is 1 x 104 CFU/ml.
5-Plex PCR Kit
|Animal Disease Standard
Diagnostic Method (QIA)
||Not Identifed same (similar) kit
||PreMix dried kit,
Detection limit of 104 cfu/ml
|Master solution Mix
* QIA: Animal, Plant and Fisheries Quarantine and Inspection Agency, Korea
Features and Benefits
Developed cooperatively with QIA, and Application of patent completed (Patented)
Less than 2-hr detection of 5 pili-type pathogenic E. coli [F4 (K88), F5 (K99), F6 (987P), F18 & F41] through one 2-step
PCR reaction in a tube.
The optimized sensitivity and specificity:
Confirmed on the 171 in-field strains owned by QIA.
Application of patented Enzyme-mediated HotStart technology: 1x104 CFU/ml of optimized sensitivity, possible exclusion of
Dried-type HotStart PCR PreMix type – Addition of template DNA and DW only required & highly reproducible result
Prevention of amplicon-induced cross-contamination by 8-MOP:
Binding of 8-MOP (8-methoxypsoralen) to DNA double-helix structure, followed by UV irradiation.
1-year warranty under -20°C.
All AccuPower® products are manufactured under ISO 9001 Quality Control System.
Multiplex PCR of pathogen detection with cDNA/complex genomic DNA/low-copy template DNA