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AccuPower® ETEC-Pili 5-Plex PCR Kit from Bioneer

Diagnosis of pathogenic 5 types of E. coli Pili gene [F4 (K88), F5 (K99), F6 (987P), F18 & F41]

Overview
Technical/Specs
Literature/Support
Ordering Information




 AccuPower® ETEC-Pili 5-Plex PCR Kit provides one-step detection of 5 types of pilis produced by ETEC E. coli in
   animals: F4 (K88), F5 (K99), F6 (987P), F18 and F41. This featured kit is comprised of all the essential requirements for
   PCR reactions including primer, salts, dNTPs, and stabilizers in one tube to the provivded ready-to-use Premix format
   product. Users only need to add template DNA and ddH20 to perform the test.

 AccuPower® ETEC-Pili 5-Pplex PCR Kit is optimized to the application in processing multiple diagnostic samples by the
   Labchip GX I (Caliper Life Sciences) automated system. Detection limit of this kit is 1 x 104 CFU/ml.


  AccuPower® ETEC-Pili
5-Plex PCR Kit
Animal Disease Standard
Diagnostic Method (QIA)
Other Kits
F4(K88) + (Detected) + Not Identifed same (similar) kit
F5(K99) + (Detected) +
F6(987P) + (Detected) +
F18 + (Detected) +
F41 + (Detected) +
Notes PreMix dried kit,
Detection limit of 104 cfu/ml
Master solution Mix

* QIA: Animal, Plant and Fisheries Quarantine and Inspection Agency, Korea


Features and Benefits

Developed cooperatively with QIA, and Application of patent completed (Patented)

 Fast response:
   Less than 2-hr detection of 5 pili-type pathogenic E. coli [F4 (K88), F5 (K99), F6 (987P), F18 & F41] through one 2-step
   PCR reaction in a tube.

 The optimized sensitivity and specificity:
   Confirmed on the 171 in-field strains owned by QIA.
   Application of patented Enzyme-mediated HotStart technology: 1x104 CFU/ml of optimized sensitivity, possible exclusion of
   false potisive.

 Great reproducibility:
   Dried-type HotStart PCR PreMix type – Addition of template DNA and DW only required & highly reproducible result
   provided.

 Prevention of amplicon-induced cross-contamination by 8-MOP:
   Binding of 8-MOP (8-methoxypsoralen) to DNA double-helix structure, followed by UV irradiation.

 High Stability:
   1-year warranty under -20°C.

 Quality Control:
   All AccuPower® products are manufactured under ISO 9001 Quality Control System.


Application

Multiplex PCR of pathogen detection with cDNA/complex genomic DNA/low-copy template DNA