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AccuPower® Bacterial Poultry 3-Plex PCR Kit from Bioneer

AccuPower® Bacterial Poultry 3-Plex PCR Kit simultaneously detects Riemerella anatipestifer, E. coli and Salmonella spp. that cause a systemic disease in poultry such as chickens, ducks, geese and turkeys.

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 Riemerella anatipestifer, E. coli and Salmonella spp. cause a systemic disease in chickens, ducks and other poultry that
   reduces weight and hatchability in them, which finally results in an enormous damage to farmers.

 E. coli is mostly related to repiratory diseasee in poultry, which may develop into sepsis, granuloma, air sacculitis,
   salpingitis and arthritis that cause tremendous economic loss.

 Riemerella anatipestifer is a nonmotile, gram-negative bacillus, and 21 serotypes have been identified to date
   —in South Korea, 1, 4, 7 and 16 serotype. It causes acute and chronic septic infectious diseases characterized by fibrous
   pericarditis, perihepatitis, air sacculitis and meningitis, which result in high mortality and weight loss in poultry.

 Salmonella causes S. Pullorum, S. Gallinarum and avian paratyphoid infection in poultry that are followed by a fall in egg
   production, fertility or hatchability, or even a death.


  AccuPower® Bacterial Poultry 3-Plex PCR Kit Standard Diagnostic Method for Animal Disease (QIA) I Company (Korea) B Company (Chile) G Company (Germany)
E. coli + (Multiplexing) - (Not detected) +( Singleplex)
Riemerella anatipestifer + (Singleplex) -(Not detected)
Salmonella spp. +(Singleplex)
Notes LoD: ~104 cfu/ml
Using a preMix dried kit
Singleplex PCR
Using a solution mix kit

* QIA: Animal, Plant and Fisheries Quarantine and Inspection Agency, Korea


Features and Benefits

Developed and patented in cooperation with QIA

 Fast detection:
   Less than 2 hour simultaneous detection of Riemerella anatipestifer, E. coli and Salmonella spp. through a 2 step PCR
   reaction in a single tube.

 Optimized sensitivity and specificity:
   Confirmed on the 60 in-field strains owned by QIA.
   By applying patented enzyme-mediated HotStart technology, achieved a sensitivity of 1x104 CFU/ml with possible
   exclusion of false potisive.

 Excellent reproducibility:
   Use of dried-type PCR PreMix only requires simple addition of template DNA and DW, which provides highly reproducible
   results.

 Prevention of amplicon-induced cross-contamination by 8-MOP addition

 High Stability:
   1-year warranty under -20 °C.

 Quality Control:
   All AccuPower® products are manufactured under ISO 9001 Quality Control System.



Application

Multiplex PCR of pathogen detection with cDNA/complex genomic DNA/low-copy template DNA